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C-terminal His-tagged Calcitonin (5 grams) and untagged GLP-1 peptide (10 grams) are accidentally mixed in 1L of 0.5M imidazole buffer. QC confirmed that there are no other impurities in the mixture. Design a suitable downstream purification scheme to obtain pharmaceutical-grade calcitonin and GLP-1, each in lyophilized form. Please include the fo
Gillian was getting married in April therefore, she was required to purchase a wedding gown and decided to purchase the gown from an online e-commerce retail outlet known as ‘The Wedding Gown World’ that advertised themselves as being wedding gown experts. Due to their website maintenance, Gillian was not able to see pictures of the gowns on th
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In Wilfred Owen’s poem, “Dulce et Decorum Est,” Owen tells a story of his horrific and gruesome experience fighting in World War I and how it has since haunted him. Owen describes a day on the battlefield that has left the soldiers mentally and physically broken and bloodied. As the soldiers are returning to the base for the night, there’s
Discuss the possible corporate governance issues relating to GM’s Board of Directors and how they could have contributed to GM’s problems. To what extent should the Board of Directors be held responsible for the safety issues? Explain. In the 1990s and early 2000s, GM had a practice of promoting the CEO to Chairman when a new CEO was appoi
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How have neobanks disrupted the traditional banking sector What are the key features & services offered by neobanks that differentiate them from traditional banks? How do neo-banks address the challenges of regulatory compliance & customer trust? cta_question_3
Question 1 On 1 May, Atkins Pte Ltd (Atkins) received brochures from Belvin Pte Ltd (Belvin), the local distributor of the “Raybon” brand of sports shoes, advertising the latest limited edition of Raybon shoes for sale at $50 per pair. Atkins wanted to get their hands on the limited-edition shoes, so they quickly sent an email to Belvin stati
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The Singapore & Malaysia governments have agreed to build a High-Speed Railway (HSR) to facilitate travel between the 2 countries In light of this, the Singapore government has approved plans for the New HSR Depot at Tuas. Train Depots are attractive targets for terror groups bent on striking transportation networks. In view of this, the
A) Identify an IT infrastructure needed for the chosen organization. Consider the following as well: What are the key components that make up an IT infrastructure for an organization? What are the business functions supported by the IT infrastructure setup? B) Identify one IT service you aim to implement in the sector to improve its service q
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Singapore will repeal Section 377A of the Penal Code, Prime Minister Lee Hsien Loong said on Sunday (Aug 21), confirming months of speculation that the government might move on to the law criminalizing sex between men. But to guard against the move triggering a drastic shift in societal norms, the Government will also amend the Constitution to pro
You are the newly hired Compensation and Benefits Manager of a non-fictitious company. You may select any real-world company with available data in Singapore. You are tasked to review and evaluate the company’s Total Rewards and its effectiveness in attracting and retaining talent. You are required to present your findings including recommendatio
