Statement on In an ideal DNA profile, both alleles in a heterozygous locus would have the same peak, Singapore

Question 1.20

Consider the following statements:

1. In an ideal DNA profile, both alleles in a heterozygous locus would have the same peak height due to the absence of preferential amplification.
2. In an ideal DNA profile, the only difference between a heterozygote and a homozygote locus would be the number of peaks seen.
3. Other than using STR markers for DNA profiling, there are other useful genetic markers that can be used for lineage tracing. One example is the mitochondrial DNA marker, which is being passed down the maternal lineage. Assuming no mutation occurs, a mother and her biological son would have the same mitochondrial DNA.
4. To account for variations between the runs of the capillary electrophoresis, the internal size standard is added to the samples.
5. DNA degradation due to exposure to UV light would be initially manifested as a loss of the smaller STR alleles.

Which of the following is correct?

• Statements 1, 2, and 3 are TRUE
• Statements 3, 4, and 5 are FALSE
• Statements 1, 3, and 4 are TRUE
• Statements 1, 2 and 4 are TRUE
• Statements 1, 3 and 5 are FALSE