The acid phosphatase test involves the transfer of a phosphate group to Bretamine Fast Black, Singapore

Question 1.8

Consider the following statements regarding examination for semen.

1. The acid phosphatase test involves the transfer of a phosphate group to Bretamine Fast Black, resulting in a purple-colouration.
2. Presence of spermatozoa indicates the presence of semen.
3. The Acid Phosphatase, Semenogelin, and Prostate-Specific Antigen (PSA) genes are encoded on the Y-chromosome, hence, they are used to test for the presence of semen.
4. Azoospermia is a possible explanation for a sample that is tested positive for Semenogelin and PSA but negative for spermatozoa.
5. The presence of semen on the vaginal swab of the victim indicates that a rape had occurred.

Which of the following is correct?

• Statements 1, 2, 3, and 5 are TRUE
• Statements 2, 3, 4, and 5 are TRUE
• Statements 1, 3, and 5 are FALSE
• Statements 1, 4, and 5 are FALSE
• Statements 1, 2, and 5 are TRUE